Enzymes and Inhibitors
New England Biolabs, Inc. NciI – 10000 units
A restriction endonuclease that recognizes the sequence CC^S_GG.
New England Biolabs, Inc. PstI – 50000 units
A restriction endonuclease that recognizes the sequence C_TGCA^G.
New England Biolabs, Inc. NEBNext Ultra™ Ligation Module – 96 reactions
This module is part of the original Ultra workflow, and is optimized for use with the NEBNext Ultra End Repair/dA-Tailing Module, for Illumina-compatible library construction. The original Ultra workflow enables library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol
New England Biolabs, Inc. PNGase A – 750 units
PNGase A cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells from N-linked glycoproteins and glycopeptides. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without (1,3)-linked core fucose residues.